About the Service

• TACMASR home page

The Tissue Acquisition and Cellular/Molecular Analysis Shared Resource provides support and pathology-related services to the Univeresity of Arizona Cancer Center and University of Arizona researchers on a fee-for-service basis. We strive to maximize research dollars through customized, cost-effective and quality-controlled services.

The Resource’s Directors and Co-Directors are board certified anatomic pathologists with many years of clinical and experimental research experience.

TACMASR Director Lisa M. Rimsza, MD, is a Professor of Pathology and has an international reputation as an expert in the diagnosis of Leukemia and Lymphoma. Dr. Rimsza is the Director of Clinical Flow Cytometry at UACM, and is the Director of the Molecular Genetic Pathology Fellowship at the University of Arizona College of Medicine. TACMASR Co-Director Charmi Patel is an assistant Professor of Pathology and is an expert in GI/Liver/ Pancreatobiliary Pathology. Dr. Patel is the Director of the Immunohistochemistry Division at UAMC. Co-Director Achyut Bhattacharyya, MD is a Professor and the Chair of the Department of Pathology and an expert in GI/Liver/Pancreatobiliary Pathology. The TACMASR Director from 200-2013, Raymond Nagle, MD, PhD, has retired.

TACMASR staff photo (Oct 2014)Each member of the TACMASR team (Doug Cromey, Carole Kepler, Betsy Dennison, Ed Abril, Dr. Charmi Patel, Dr. Lisa Rimsza, Tina Liu, Kathy McDaniel, Missing: Bill Meek and Estella Chapa) brings many years of experience and expertise to the shared service.

Priority of Access

Preference in scheduling services is given to UACC Members and Investigators with peer reviewed and competitively funded grants, to UA Investigators and then to external entities.

Biohazardous samples

All biological materials are potential biohazards, some more than others.

As a courtesy, please include relevant information about potential biohazardous samples or conditions on the Request Form.

Notify the lab staff if your samples present any undue biological hazard.

TACMASR does not accept

  • Radiolabeled material
  • Fresh material with known infectious agents (AIDs, hepatitis, etc)
  • Fresh tissue without making prior arrangements with staff
  • Cell pellets on Fridays without prior arrangements

Resources

TACMASR is equipped with technology and reagents to perform routine histology including:  TissueTek tissue processor, embedding station, two rotary microtomes, Microm HM550 cryostat, a Benchtop Solvent Recycler (CBG Biotech) and refrigerators/freezers. 

Tissue specimens are handled in a ThermoScientific Shandon Tissue Grossing Station. Blood and urine are centrifuged in a Sorvall Legend RT Refrigerated Benchtop centrifuge and aliquotted in a NuAire Biological Safety Cabinet Class II Type A/B3.  A Shandon Cytospin 2 is used to prepare cytospin cell preps. Two rolling carts containing equipment and supplies to fix and snap freeze tissue are available. Frozen biospecimens are stored in four -80°C freezers located in the basement of the Salmon Building. This room is equipped with backup emergency power and an emergency alarm system which is monitored by UA Facilities Management. 

The majority of IHC assays are performed on the Discovery XT Automated Staining platform for IHC and ISH (Ventana Medical Systems, Inc, Tucson, Ariz.). Equipment for manual staining immunostaining is also available.

[NOTE: currently not working] The Arcturus VeritasTM Laser Capture Laser Microdissection instrument is available for use by investigators. TACMASR provides technical support to prepare samples for LCM and trains users to harvest RNA, DNA and protein by LCM to address issues of tissue heterogeneity.  A Qiagen BioRobot EZ1 for harvesting DNA from blood samples is also available.

Photomicrography and molecular imaging equipment includes: 

  • An Olympus BX50 microscope and CellSens DP72 imaging camera/software for both light and fluorescence viewing.

  • BioImagene iScan Coreo Au slide scanning and quantitation instrumentation.


Review an updated TACMASR Equipment List

Outside investigators and commercial biotech companies please contact the TACMASR Staff  

Review a list of 2003-2008 TACMASR Select Publications