Resources

Our current resources include a variety of mass spectrometers interfaced to nanoHPLC, 1D and 2D SDS-PAGE equipment, imagers and scanners, and protein purification hardware.

List of Current Resources

LCQ Classic (1998) (Financial support provided by NIEHS ES06694)

3D quadrupole ion trap mass spectrometer
ESI (nanospray)
MS and MS/MS scans
Nano LC-MS/MS system
Flow rates 250 nanoLit/min
Michrom MAGIC 2002 dual pump
UV detector available with higher flows on LC
Alcott autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Suited for proteomic applications
Peptide sequencing (confirmation)
Protein identification (gel or solution)
Sensitivity 300 fmol standard digest (bovine serum albumin)

LCQ DECA XP+ (2003) (Financial support provided by the University of Arizona, Arizona Cancer Center and University of Arizona College of Pharmacy)
3D quadrupole ion trap mass spectrometer
ESI (nanospray)
MS and MS/MS scans
Nano LC-LC-MS/MS system
Flow rates 250 nL/min
Michrom Paradigm MS4 quaternary pump HPLC
Surveyor autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Fused silica (250 um ID x 365 um OD) strong cation exchange (SCX) Whatman Partisphere self packed to 7 cm
Dedicated to Multidimensional Protein Identification Technology (MudPIT) or LC-LC-MS/MS
Whole proteome analyses
Solution protein mix
Gel separated protein mix (whole lane)
Sensivity 150 fmol standard digest (bovine serum albumin)

LCQ DECA XP+ (2002) (Financial support provided by BIO5 Institute)
3D quadrupole ion trap mass spectrometer
ESI (nanospray)
MS and MS/MS
Nano LC-MS/MS system
Flow rates 250 nanoLit/min
Surveyor quaternary pump HPLC
Surveyor autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Protein identification applications
Sensitivity 100 fmol standard digest (bovine serum albumin)

LTQ (2006) (Financial support provided by Ventana Medical, Tucson AZ)

2D quadrupole ion trap mass spectrometer
ESI (nanospray)
MS and MS/MS
Nano LC-MS/MS system
Flow rates 250 nanoLit/min
Michrom Paradigm MS4 quternary pump HPLC
TSP 3000 autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Protein identification applications
Sensitivity 30-50 fmol standard digest (bovine serum albumin)

LTQ (2006) (Financial Support provided by NIH U54-A1065359)
2D quadrupole ion trap mass spectrometer
ESI (nanospray)
MS and MS/MS
Nano LC-MS/MS system
Flow rates 250 nanoLit/min
Surveyor quaternary pump HPLC
Surveyor autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Protein identification applications (A)
Multidimensional Protein Identification Technology (MudPIT) or LC-LC-MS/MS(B)
Fused silica (250 um ID x 365 um OD) strong cation exchange (SCX) Whatman Partisphere self packed to 5 cm
Whole proteome analyses
Solution protein mix
Gel separated protein mix (whole lane)
Switch between (A) and (B) design as dictated by necessity
Sensitivity 30-50 fmol standard digest (bovine serum albumin)

ABI 4000 QTRAP (2006) (Financial support provided by NIH 1S10RR022384-01)

Hybrid triple quadrupole linear ion trap mass spectrometer
ESI and nanospray
MS and MS/MS
Single ion monitoring (SIM), multiple reaction monitoring (MRM), neutral loss scan
Nano LC-MS/MS system
Flow rates 250 nanoLit/min
LC Packings Ultimate dual pump HPLC
Dionex FAMOS autosampler
Fused silica (100 um ID x 365 um OD) reversed phase (RP) C18 columns (tip 3-5 um); self packed to 7 cm
Small molecule analyses
Protein characterizarion
Targeted MS/MS and MRM workflow

Waters Q-TOF Premier (2008) (Financial support provided by NIEHS ES06694, the University of Arizona and the Arizona Cancer Center)
ESI and nanospray
MS and MS/MS
Nano LC-MS/MS system
Waters nanoACQUITY
Label free quantitation in liquid phase
Ion Accounting software

Waters/Micromass MALDI-LR (2002) (Financial support provided by a private donor gift to the Arizona Cancer Center, ES 06694 and the University of Arizona College of Pharmacy)
MALDI ionization
MS
Linear mode
Reflectron mode
Protein mass measurements
5 pmol/uL detection limit
Resolution 100-500
Peptide mass mapping
50-100 fmol/uL detection limit
Resolution 10000
Waters Protein Linx search engine

 

ABI Voyager DE-STR (2004) (Financial support provided by the University of Arizona and the College of Pharmacy)

MALDI ionization
MS
Linear mode
Refletron mode
Limited post-source decay (PSD)
Protein mass measurements
1 pmol/uL detection limit
Resolution 100-500
Peptide mass mapping
50 fmol/ul
Resolution 20000
MASCOT search engine

Genomic Solutions ProPicII (2007) (Financial support provided by NIEHS ES06694)
Imaging of coomassie, silver, sypro gels
Spot picking in 96 well plate
Gel image comparison software (Non Linear)
Compatible with Typhoon 9400 imaged gel (DIGE) and pick list

 

 

 

 

 

Genomic Solutions ProGest and ProMS (2003) (Financial support provided by ES06994)

Automated in-gel and solution digestion (trypsin, chymotrypsin, etc)
Automated MALDI-LR target plate spoting

 

 

 

 

 

Perkin Elmer Multiprobe (2002) (Financial support provided by the BIO5 Institute)

Liquid handling robot for automated digestion

 

 

 

 

 

 

AKTA FPLC (2003) (Financial support provided by the BIO5 Institute)

 

 

 

 

 

 

 

Michrom HPLC (2007) (Financial support provided by Ventana Medical Systems, Tucson, AZ)

 

 

 

 

 

 

 

GE Healthcare Typhoon 9400 Imager (2003) (Financial support provided by the BIO5 Institute

Imaging of cy2, cy3 and cy5 labelled 2D SDS-PAGE gels for DIGE applicationsDecyDer v 6.5 analysis software

 

 

 

 

 












 

 

BioRad 1D and 2D SDS-PAGE equipment (2000-2007)

1D SDS-PAGE on 7 cm mini gels and 11 cm Criterion gels
Coomassie, silver and Sypro ruby stains
2D SDS-PAGE on 7 cm mini gels and 11 cm Criterion gels
variety of IPG strips for IEF in the 1st dimension (pH 3-10, 4-7, etc)

 

 

 

 

 

 

 

 

 

 

 

Sutter P200 Capillary puller (2000)

Fabrication of pulled fused silica columns (tips to 5 um opening) for nano C18 or SCX columns