A variety of research projects from members of the AZCC are highlighted below
Cancer Biology and Genetics Program
Zhang J. Tsaprailis G. Bowden GT. Cancer Res. 68:1046-1054, 2008.
- Anti-apoptotic Bcl-XL crucial for cell survival and implicated in differentialtion and development
- Overexpression in certain cancers
- Stability dependent on on 3’-UTR of Bcl-XL mRNA following UVA irradiation
- Mechanism of stability unclear
- LC-MS/MS used to identify nucleolin as one of protein binding to 3’-UTR of Bcl-XL mRNA following UVA irradiation
Gastrointestinal Cancer Program
Uemura T. Yerushalmi HF. Tsaprailis G. Stringer DE. Pastorian KE. Hawel L. Byus CV. Gerner EW. J. Biol. Chem. 283:26428-26435, 2008.
- Understanding polyamine transport may be important for new strategies of cancer treatment and chemoprevention
- uptake/export role in colon cancer
- Chinese hamster ovary cells sensitive to the diamine putrescine (CHOS)
- Chinese hamster ovary cells tolerant to the diamine putrescine (CHOT)
- Increased export of putrescine
- Protein profiling changes in CHOS and CHOT cells via 1D and 2D SDS-PAGE and LC-MS/MS
- SLC3A2 over expressed in CHOT
- Proteomic finding led to knockout expts, use of human colon cancer cells
- Knockout SLC3A2: inhibition by putrescine
- Activated K-RAS cells: decreased SLC3A2 - regulation control
- Model of transport proposed
Therapeutic Development Program
Leinweber BD. Tsaprailis G. Monk TJ. Lau SS. JASMS 20(1):89-95, 2009.
- MALDI-TOF MS used to image protein localization and profiles in tissues
- Novel techniques developed allows MALDI-TOF MS signals up to 50kDa
- Eker (Tsc-2+/Ek) rats treated with TGHQ or saline (TGHQ carcinogen)
- MALDI-TOF MS tissue imaging used to profile proteins in regions of interest (ROIs) TGQH-associated tumors
- LC-MS/MS used to identify protein consistent with MALDI-TOF MS spectra
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