Proteomics Core Shared Service

The overall objective of the Proteomics Shared Service (PSS) is to provide The University of Arizona Cancer Center investigators with a dedicated facility and expertise in analyzing proteins for their identity, quantity and function via state-of-the art modern mass spectrometry and peripheral analytical instrumentation.

Over the past funding cycle, the PSS has performed proteomics analyses for Cancer Center members belonging to 5 different programs (units of service).

 

We offer a variety of proteomics services and tools to members of the Cancer Center.

  • Gel Electrophoresis - used to separate proteins in a gel matrix
    • 1D SDS-PAGE on 7 and 11 cm formats
      • separation by MW
      • coomassie, silver and Sypro Ruby images

  • 2D SDS-PAGE on 7 and 11 cm formats
    • separation by pI and MW
    • coomassie, silver and Sypro Ruby images

  • Differential In-Gel Electrophoresis (DIGE) - expression analysis of complex protein samples
    • Cy3, Cy5 and Cy2 labelling
    • separation by pI and MW
    • DeCyder expression analysis
    • custom reports of expression differences
    • statistics

  • Protein MW determination
    • ESI or MALDI ionization

  • LC-MS/MS
    • Digestion of protein in solution or in-gel
    • nano HPLC introduction of peptides into mass spectrometer
    • tandem mass spectrometry (MS/MS) peptide sequencing
    • database searching
      • Sequest, X!Tandem, MASCOT outputs
      • SCAFFOLD visualization package
      • secure on-line results on www.protbase.org

  • LC-LC-MS/MS
    • as LC-MS/MS but with dual nanoLC separation (Strong Cation Exchange and Reversed Phase chromatography)

  • Albumin Depletion
    • 12-most abundant protein depletion using FPLC
  • Protein Purification
    • combination of HPLC, gel filtrationa and FPLC (inquire with Core personnel)
  • Phosphopeptide Enrichment
  • Sample concentration and clean-up

Please click here for a full list of prices

Prices are subject to change without notice and set by the University of Arizona Financial Services Office.