Cell Culture Services

The EMSS provides laboratory support for all Cell Culture needs.

 

Large scale culture
 

The EMSS has a large repository of mycoplasma-tested and cell-line authenticated cells available for in vivo or in vitro-leading-to-in vivo research.  The EMSS can grow these or other established cell lines and provide all necessary cell culture products and mycoplasma screening.

EMSS provides some human optical reporter cell lines that have been transfected with GFP, RFP and luciferase for experimental purposes. These cell lines include breast, pancreatic and colon cancers and are bolded; Cell Lines & Tumor Types Listed Below.
 

Mycoplasma screening
 

The EMSS regularly tests all cell lines grown in-house for mycoplasma contamination and offers weekly testing for other investigators.  Regular screening of cell lines for mycoplasma is extremely important. This is especially true when accepting any cell line from an outside source. Mycoplasma can alter virtually every property measured in cell cultures. Working with contaminated cultures may nullify all results. Recommended treatment methods can be provided in cases of mycoplasma contamination.


Consultation & training
 

Consultation and training sessions are available. Session topics often range from how to solve contamination problems, to questions about cell growth, to cell culture techniques. Contact us about your topic interests and scheduling opportunities.


Bank of tumor cell lines

 

(coming soon)
 

Patient-derived cell line

 

The EMSS can derive cell lines from patient samples.  

Cell line authentication

 

In addition to maintaining a selection of authenticated cell lines, the EMSS can authenticate cell lines.  Journals are enacting more stringent guidelines to ensure the accuracy of the research they publish.  The EMSS can test a cell line from a pellet or grow and sample a pellet for testing and report the results.

To check on the availability of cells, Contact us
Investigators receiving cell lines must complete a Cell Line Waiver Form.

Human, Murine, & Rat Tumor Models

The model of choice can be used, and assistance is available to help you select the most appropriate model for the investigation agent you are using. Specially requested cell lines or tumor chunks can be brought in-house if a desired model is not listed below. Note that the human optical reporter cell lines are bolded. 

                                  Human Tumor Xenografts

   Tumor Types

                       Cell Line Name

Breast

BT-474, MCF7/S, MDA-MB-231, ZR-75-1, MCF7/TAMR-1, MCF7/Mitox, MCF7/D40, MDA-MB-453, MCF7/CMV-Luc, MCF7/eGFP, MDA-MB-231/CMV-Luc, MDA-MB-231/eGFP, MDA-MB-231/eRFP

Burkitt's Lymphoma

Ramos

Cervix

HeLa, Ca Ski, SiHa, C-33A

Colon

CaCo-2, HCT 116, HT-29, SW480, SW620, HCA-7, HCT-116/eRFP

Epidermis

A-431

Fibrosarcoma

HT-1080

Gastrointestinal Stromal Tumor

GIST882

Glioblastoma

U-87 MG

Head & Neck

HN5

Leukemia

HL-60, K562/S, K562/MDR, KG-1, OCI-AML3

Liver

SK-HEP-1

Lung-NSCLC

A549, MV522, NCI-H1299, NCI-H460, NCI-H1975, NCI-H1993

Lung-SCLC

SHP-77

Lymphoma

Daudi, Raji, Granta 519, Granta 4,
KARPAS-299, CA46, U2932

Melanoma

A-375, DH903, JH1308, KD1592, PS1273, WM1791C, LOX IMVI, SB-CL2, MDA-MB-435

Myeloma

8226/S, 8226/V, 8228/Dox40, ARH-77, ARHD60

Neuroblastoma

SK-N-SH

Osteosarcoma

Saos-2, U-2 OS

Ovarian

A2780, A2780/ADR, NIH:OVCAR-3

Pancreatic

AsPC-1, BxPC-3, CFPAC-1, HPAF-II,
MIA PaCa-2, MIA PaCa-2/LucE, PANC-1, SU.86.86, Capan-2

Prostate

DU 145, LNCaP, PC-3, VCaP

Renal

786-O, A-498, ACHN, Caki-1

Sarcoma- Ewing's

CHP-100

Uterine Epithelial

RL 95.2

Mouse Tumor Xenografts

Melanoma

B16-F0

Rat Tumor Xenografts

Breast

13762NF

Glioblastoma

C6

New University or Cancer Center Clients, please Contact Us.
Investigators receiving cell lines must complete a Cell Line Waiver Form.