The EMSS uses in vivo models to complete preclinical research and evaluate anti-cancer agents for efficacy in prevention, intervention and regression. We perform these studies in both xenograft and genetically engineered mouse (GEM) models. The EMSS is a full-service facility, and our team provides technical and scientific expertise to investigators regarding the choice of in vivo model, study design and data collection.
The EMSS maintains a colony of SCID mice to help reduce the cost of xenograft studies. The service can perform xenograft studies from cell culture to tissue harvest. Our team is certified to perform surgeries, irradiation, fluorescence and bioluminescence imaging.
Implantation options include:
• Subcutaneous injection of tumor cells (flank, mammary fat pad, dorsal)
• Orthotopic injection or implantation
• Window chamber
• Intracardiac injection
• Intraosseus injection
All xenograft options can also be performed in any immunodeficient or GEM models.
We can test Maximum Tolerated Dose in novel compounds. These studies often lead to subsequent pharmacokinetics and then xenograft or GEM model studies.
Acute Toxicity and Pharmacokinetic Studies
Important aspects of early preclinical drug development are acute toxicity and pharmacokinetic studies. The EMSS Team offers basic services in bioavailability, pharmacokinetics, and toxicity analysis.Toxicology studies can include any or all of the following:
• Timed agent dosing and sampling
• Tissue and/or organ preservation
• Plasma and/or serum sampling
• Histology and pathology
• Complete Blood Counts with differentials
Pharmacokinetic sampling can be designed as a stand alone experiment, or it can be included into your therapeutic agent efficacy design. The EMSS works in collaboration with the Analytical Core Shared Service for design and analysis of the pharmacokinetic samples.
The EMSS can also perform toxicity studies in rat models.
Samples harvested from in-vivo studies are sent to the Tissue Acquisition and Cellular/Molecular Analysis Shared Service or to the UAC Pathology lab for processing and analysis.