The EMSR uses in vivo models to complete preclinical research and evaluate anti-cancer agents for efficacy in prevention, intervention and regression. We perform these studies in both xenograft and genetically engineered mouse (GEM) models. The EMSR is a full-service facility, and our team provides technical and scientific expertise to investigators regarding the choice of in vivo model, study design and data collection.
The EMSR maintains a colony of SCID (severe combined immune deficient) mice to help reduce the cost of xenograft studies; however, any strain of mouse can be utilized. The service can perform xenograft studies from cell culture to tissue harvest. Our team is certified to perform surgeries, irradiation, fluorescence and bioluminescence imaging.
Implantation options include:
• Subcutaneous injection of tumor cells (flank, mammary fat pad, dorsal)
• Orthotopic injection or implantation
• Window chamber
• Intracardiac injection
• Intraosseus injection
All xenograft options can also be performed in any mouse model.
We can test Maximum Tolerated Dose in novel compounds. These studies often lead to subsequent pharmacokinetics and then xenograft or GEM model studies.
Acute Toxicity and Pharmacokinetic Studies
Important aspects of early preclinical drug development are acute toxicity and pharmacokinetic studies. The EMSR Team offers basic services in bioavailability, pharmacokinetics, and toxicity analysis.Toxicology studies can include any or all of the following:
• Timed agent dosing and sampling
• Tissue and/or organ preservation
• Plasma and/or serum sampling
• Histology and pathology
• Complete Blood Counts with differentials
Pharmacokinetic sampling can be designed as a stand alone experiment, or it can be included into your therapeutic agent efficacy design. The EMSR works in collaboration with the Analytical Core Shared Service for design and analysis of the pharmacokinetic samples.
The EMSR can complete all mouse experimentation techniques in rat models.
Samples harvested from in-vivo studies can be sent to the Tissue Acquisition and Cellular/Molecular Analysis Shared Service, the UAC Pathology lab, or the investigator’s lab for processing and analysis.
Disclaimer: The Experimental Mouse Shared Resource will perform experiments outlined in the investigator approved experimental designs with the highest care and quality. The Experimental Mouse Shared Resource is not responsible or liable for any claims, loss or damage arising from the quality of the material (cell lines etc.) or the experimental design (drug doses, frequency, etc.) provided by the Investigator.