Developed / Implemented Assays

Below is a list of assays developed or implemented in the Analytical Chemistry.

Analyte

Sample Type

Instrument

Selenium

Plasma 

AA 

Glyoxal, Methyl glyoxal

Cell extract

GC-MS

Malondialdehyde

Cell extract

GC-MS

Tea Catechins

Plasma; Urine 

HPLC-EC 

 4-HPR (N-(4-hydroxyphenyl)-retinamide)

Plasma; Tissue

HPLC-FLD 

Arginine/Ornithine

Intestine; Colon Tissue

HPLC-FLD 

Carotenoids

Plasma; Skin 

HPLC-FLD 

Chlorophyll 

Colon Tissue 

HPLC-FLD

Dextromethorphan (DM)/DM metabolites

Urine 

HPLC-FLD 

DFMO (Alpha-difluoromethylornithine)

Plasma; Skin 

HPLC-FLD

gamma-Glutamylcysteine 

Cell extract 

HPLC-FLD 

Nitrite

Cell culture media

HPLC-FLD

Pentosidine

Plasma

HPLC-FLD

Polyamines

Plasma; Tissue

HPLC-FLD 

Retinols

Plasma; Skin 

HPLC-FLD

Targretin

Plasma

HPLC-FLD 

Tocopherol/Tocopherol succinate

Plasma; Tissue 

HPLC-FLD

AMP423

Formulation Preparation

HPLC-MS 

Bile acids

Fecal Water; Plasma; Intestinal fluid

HPLC-MS 

Resveratrol

Plasma; Tissue

HPLC-MS/MS 

8-Hydroxy-2'-deoxyguanosine

Urine; DNA 

HPLC-MS/MS 

8-Isoprostaglandin F2 alpha 

Urine; Plasma

HPLC-MS/MS

Acetylcholine

Mouse cortex

HPLC-MS/MS

Alpha-tocopheryloxyacetic acid

Plasma

HPLC-MS/MS

Antalarmin 

Plasma 

HPLC-MS/MS 

Bile acids (parent bile acids and taurine and glycine conjugates)

Gastric fluid

HPLC-MS/MS 

Buspirone 

Plasma 

HPLC-MS/MS 

Carbendazim 

Plasma 

HPLC-MS/MS 

Cortisol/Cortisone 

Saliva

HPLC-MS/MS 

Dexmedetomidine

Urine

HPLC-MS/MS 

DIM (diindolylmethane)

Urine

HPLC-MS/MS

Diphenidol

Cell culture media

HPLC-MS/MS

DITPA (3,5-diiodothyropropionic acid) 

Plasma 

HPLC-MS/MS

Dopamine

Mouse Cortex

HPLC-MS/MS

DPC0046

Plasma

HPLC-MS/MS

Epinephrine

Mouse cortex

HPLC-MS/MS

Estradiol/estrone

Plasma; urine

HPLC-MS/MS

Fatty acids

Plasma

HPLC-MS/MS

GABA (gamma-aminobutyric acid)

Mouse Cortex; Urine

HPLC-MS/MS 

Glutamate

Mouse Cortex

HPLC-MS/MS 

HETEs (hydroperoxyeicosatetraenoic acid)

Lung Tissue 

HPLC-MS/MS

Isothiocyanate-glutathione conjugates

Cultured cells 

HPLC-MS/MS 

Melphalan/Melphalan analogues

Dosing Preparation 

HPLC-MS/MS

Metformin

Plasma; Cell culture media; Tissue

HPLC-MS/MS 

Midazolam/ Midazolam Metabolites

Plasma

HPLC-MS/MS

Nitotinic acid/Nicotinamide

Plasma 

HPLC-MS/MS

Norepinephrine

Mouse cortex

HPLC-MS/MS

Omeprazole/Metabolites

Plasma

HPLC-MS/MS

Pemetrexed (Alimta®)

Plasma; IP fluid

HPLC-MS/MS

Phenethylamine

Urine

HPLC-MS/MS

PMIP (therapeutic peptide)

Plasma

HPLC-MS/MS 

Quercetin

Plasma; skin

HPLC-MS/MS

Radicicol

Plasma

HPLC-MS/MS

Rapamycin

Whole blood

HPLC-MS/MS

Taurine

Urine

HPLC-MS/MS

Testosterone

Plasma

HPLC-MS/MS 

Sulindac/Sulindac metabolites

Plasma; Breast Fluid; Skin 

HPLC-MS/MS 

Thyroxine/Triiodothyroxine

Plasma 

HPLC-MS/MS 

UA8967

Plasma

HPLC-MS/MS

Withaferin analogues

Plasma

HPLC-MS/MS 

Wortmannin analogues

Plasma; Bile

HPLC-MS/MS

Limonene 

Plasma; tissue 

HPLC-MS/MS; GC-MS 

Celecoxib 

Plasma 

HPLC-UV 

Cortisol/6-Hydroxycortisol 

Urine

HPLC-UV 

Curcumin

Plasma; Urine; Skin 

HPLC-UV

Gemcitabine Triphosphate

Blood Lymphocytes; Cell Extract

HPLC-UV

Imexon

Plasma 

HPLC-UV 

NPA-093

Plasma

HPLC-UV

Psorospermin methyl ether

Plasma

HPLC-UV 

Gemcitabine 

Plasma; Tissue; Cell Extract 

HPLC-UV; HPLC-MS/MS

PHT-427

Plasma; Tissue

HPLC-UV; HPLC-MS/MS

PX-12 

Plasma 

HPLC-UV; HPLC-MS/MS

Caffeine/Caffeine metabolites 

Plasma 

HPLC-UV;HPLC-MS/MS

Losartan/Losartan Metabolites

Urine; Plasma

HPLC-UV;HPLC-MS/MS

 

In addition to the listed methods, the Analytical Chemistry Shared Resource can develop analytical procedures for determination of novel chemicals in biological samples and adapt additional published analytical procedures.