Thomas Doetschman, PhD

Professor, BIO5 Institute and Department of Cellular & Molecular Medicine

E-Mail Address: 
Phone Number: 
(520) 626-4901
Fax: 
(520) 626-7600
Short Bio: 

1968, BA, Social Studies, University of Chicago

1980, PhD, Biochemistry & Biophysics, University of Connecticut

1980-83, Postdoc, Muscle Development Swiss Federal Institute of Technology, Zurich, Switzerland

1983-86, Postdoc, Embryonic Stem Cells, Friedrich Miescher Institute, Max Planck Institute, Tubingen, Germany

1987-88, Postdoc, Gene Targeting, Dept. Genetics, University of Wisconsin

1988-2006, Assist Professor to Professor, Dept Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati

1990-2006, Founder and Sr. Advisor to Gene Targeting Core Facility, U of Cincinnati

1997-2002, Adjunct Professor, Institute of Molecular and Cell Biology, National University of Singapore.

2006-Pres, Professor, BIO5 Institute and Dept. Cellular & Molecular Medicine, University of Arizona

2006-Pres, Founder and Director, Genetically Engineered Mouse Models (GEMM) Core Facility

2007-Pres, Co-Director, Experimental Mouse Shared Resource (EMSR)

 

Research Information
Research Program: 
3. Cancer Biology
Member Status: 
Member
Year of Membership Acceptance: 
2006
Summary of Research Activity: 

TGFβ signaling is disrupted in up to a quarter of all human colon cancer, and inflammation often accompanies human colon cancer. We are using next generation sequencing to investigate the changes in colon mucosa/microbiome interactions that occur in a TGFβ-signaling-deficient mouse model of inflammation-associated colon cancer. Another study utilizes a nuclear green fluorescent protein indicator mouse for isolation and whole exome analysis of singe nuclei from cells at the initiating stages of mouse prostate and pancreatic cancer. This indicator mouse has applicability to a wide range of cancer models.

Selected Publications: 

Doetschman T. (2011) GI GEMs: Genetically engineered mouse models of gastrointestinal disease. Gastroenterology 140:380-385.

Doetschman T, Sholl A, Chen HDR, Gard C, Hildeman D, Bommireddy R. (2011) Divergent effects of calcineurin Aβ on regulatory and conventional T-cell homeostasis. Clin. Immunol. 138:321-330.

Bommireddy R, Babcock GF, Singh RR, Doetschman T. (2008) TGFbeta1 deficiency does not affect the generation and maintenance of CD4(+)CD25(+)FOXP3(+) putative T(reg) cells, but causes their numerical inadequacy and loss of regulatory function. Clin. Immunol. 127:206-213.

Bommireddy R, Doetschman T. (2007) TGFbeta1 and T(reg) cells: alliance for tolerance. Trends Mol. Med. 13:492-501.

Kaiser S, et al. (2007) Transcriptional recapitulation and subversion of embryonic colon development by mouse colon tumor models and human colon cancer. Genome Biol. 8:R131.

Engle SJ, Ormsby I, Pawlowski S, Boivin GP, Croft J, Balish E, Doetschman T. (2002) Elimination of colon cancer in germ-free Transforming Growth Factor beta 1-deficient mice. Cancer Res. 62:6362-6366.

Engle SJ, Hoying JB, Boivin GP, Ormsby I, Gartside PS, Doetschman T. (1999) Transforming growth factor beta1 suppresses nonmetastatic colon cancer at an early stage of tumorigenesis. Cancer Res. 59:3379-3386.

Shull MM, Ormsby I, Kier AB, Pawlowski S, Diebold RJ , Yin M, Allen R, Sidman C, Proetzel G, Calvin D, Doetschman T. (1992) Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease. Nature 359:693-699.

Doetschman T, Gregg RG, Maeda N, Hooper ML, Melton DW, Thompson S, Smithies O. (1987) Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330:576-578.

 

Collaborative Research: 

Microbiome/Colon Mucosal Epithelium interactions are being investigated by next generation sequencing in collaboration with Bioinformaticist Dr. Bonnie Hurwitz, Dept of Agricultural and Biosystems Engineering.

Determination of whole expressome from single fluorescence-sorted nuclei from cells at the initiation stage of pancreatic and prostate cancer cells is being done in collaboration with Dr. David Galbraith, Therapeutic Development Program, and Dr. Ron Heimark, Cancer Biology.

 

What prompted you to get into cancer research?: 

Functional analysis of TGFβ signaling in mice with genetically engineered null mutations in the 3 TGFβ ligands revealed a colon tumor suppressor role for TGFβ1.

Academic Information
Doctorate: 
University of Connecticut, 1980, Biochemistry & Biophysics
Undergraduate School: 
University of Chicago, 1968, Social Sciences