Josef Vagner, PhD

Associate Research Professor

E-Mail Address: 
Phone Number: 
(520) 626-4179
Short Bio: 

July 2005 – present
University of Arizona, Bio5, Tucson, AZ, Associate Research Professor, Director of Ligand Discovery Lab; Advanced Research Institute for Biomedical Imaging (member)
High throughput synthesis and screening of diverse chemical libraries.
Design of ligands for disease diagnosis and therapy.
Design and synthesis of labeled biomarkers and pharmaceuticals.

May 2002 – June 2005
University of Arizona, Bio5, Tucson, AZ, Assistant Research Professor (Organic Chemist)
Scientific advisers: Prof. Robert J. Gillies and Prof. Victor J. Hruby
The design and solid-phase synthesis of multimeric ligands for binding to the combination of epitopes specific to human cancer cells.

December 1998 – September 2001
SIDDCO, Tucson, AZ, Scientific Fellow 5 (Synthetic Organic Chemist/Principal Scientist)
The design and synthesis of combinatorial libraries in a format suitable for high throughput screening as potential drugs. The development and optimization of reactions and scaffolds on solid-phase supports (the protocols to be used in the production of combinatorial libraries).

November 1995 – September 1998

Novo Nordisk A/S, Copengagen, Denmark, Research Chemist
The design and synthesis small combinatorial libraries – arrays of organic compounds, and multiple synthesis in automatic devices. The development of reactions on solid-phase support, multi-step organic syntheses, purifications and analyses of products.

October 1992 - October 1995
University of Minnesota, Department of Chemistry, Minneapolis, USA; Postdoctoral Fellow
Scientific adviser: Prof. George Barany
The solid-phase peptide synthesis involving the synthesis of defensins, orthogonal protection schemes, acid-labile handles and protecting groups. The combinatorial chemistry for synthesis of non-peptide libraries.

Research Information
Research Program: 
2. Cancer Imaging
Member Status: 
Member
Summary of Research Activity: 

Currently, I am leading the Ligand Discovery Lab (LDL) at Bio5, University of Arizona. The current research projects deal with the development of ligands, inhibitors, and diagnostic probes in collaboration with the AZCC, UNMCC, TGen, Department of Radiology and Department of Biochemistry.

The Ligand Discovery Laboratory (LDL) is a complex chemistry center supporting investigators with libraries of chemical compounds and provides their screening.
 
The primary objective of the LDL is the design, synthesis, purification, characterization and screening of arrays of unique compounds for drug discovery. The LDL uses the following technologies:
  • Solid-phase synthesis (small organic compounds, biopolymers)
  • High throughput synthesis (96-well plates)
  • One-bead-one-compound (OBOC) library synthesis
  • Rational design of small molecule ligands, multimeric ligands, peptidomimetics
  • Lanthanide-based time-resolved fluorometry in vivo binding assay
  • In vivo cell-adhesion-to-bead assay for OBOC library
  • HT synthesis of labeled probes (fluorescent, DOTA-Eu)
  • Logistics and QC
Lead structures can undergo multiple development cycles. At each cycle, we will look for improvement in binding affinity, selectivity, physico-chemical properties (solubility, stability), and suitability for imaging, which is the most important parameter. Structural, analytical and storage data are entered into a database (ChemOffice, CambridgeSoft) which is compliant with the Molecular Libraries and Imaging Initiative (MLII) database.
Selected Publications: 

Vagner, J., Barany, G., Lam, K.S., Krchnak, V., Sepetov, N.F., Ostrem, J.A., Strop, P., and Lebl, M., Enzyme-mediated spatial segregation on individual polymeric support beads: application to generation and screening of encoded combinatorial libraries. Proceedings of the National Acadademy of Science of the U.S.A., 93, 8194-8199 (1996).

Cowell, S.M.; Gu, X.; Vagner, J.; Hruby, V.J. Intelligent design in combinatorial chemistry: use of designed peptide libraries to explore secondary and tertiary structures in peptides and proteins. Methods in Enzymology, 369 (Combinatorial Chemistry, Part B), 288-297 (2003).
 
Handl, H.L.; Vagner, J.; Han, H.; Mash, E.; Hruby, V.J.; Gillies, R.J.  Hitting multiple targets with multimeric ligands. Expert Opinion on Therapeutic Targets, 8, 565-586 (2004).
 
Vagner, J., Handl, H., Gillies, R.J., Hruby, V.J. Novel strategy based on multimeric ligands for drug delivery and imaging: homooligomers of a-MSH. Bioorganic & Medicinal Chemistry Lett. ,14, 211-215 (2004).
 
Handl, H.L., Vagner, J., Yamamura H.I., Hruby, V.J., Gillies, R.J. Lanthanide-based time-resolved fluorescence of in cyto ligand-receptor interactions. Analytical Biochemistry, 330, 242-250 (2004).
 
Vagner, J., Handl, H.L., Monguchi, Y., Jana, U., Begay, L., Mash, E. A., Hruby, V.J., Gillies, R.J. Rigid linkers for bioactive peptides. Bioconjugate Chemistry, 17,1545-1550 (2006).
 
Mayorov, A.V. Cai, M. Palmer, E.S., Dedek, M.M., Cain, J.P., Van Scoy, A.P., Tan, B. Vagner, J., Trivedi, D., Hruby, V.J., Structure-Activity Relationships of Cyclic Lactam Analogues of a-MSH. Targeting the Human Melanocortin-3 Receptor.Journal of Medicinal Chemistry 51, 187-195 (2008).
 
Vagner J., Xu L., Handl H.L., Josan J.S., Morse D.L., Mash E.A., Gillies R.J., and Hruby V.J., Heterobivalent Ligands Crosslink Multiple Cell-Surface Receptors: The Human Melanocortin-4 and d-Opioid Receptors. Angewandte Chemie Intl. Ed. 47, 1685-1688 (2008).
 
Black, K.C., Kirkpatrick, N.D., Troutman, T.S., Xu, L., Vagner, J., Gillies, R.J., Barton, J.K., Utzinger, U., Romanowski, M. Gold nanorods targeted to delta opioid receptor: plasmon-resonant contrast and photothermal agents. Molecular Imaging 7, 50-57 (2008).
 
Vagner, J., Qu, H., Hruby, V.J.  Peptidomimetics, a synthetic tool of drug discovery. Current Opinion in Chemical Biology 12(3), 292-296 (2008).
Collaborative Research: 

The LDL proram relies on an existing information flow from TGen, and collaborations with other groups:

Victor J Hruby (UA,pancreatic cancer, melanoma),

Gene Mash (UA,pancreatic cancer, melanoma),

Bob Gillies (Moffitt, pancreatic/breast cancer and melanoma),

Bill Monfort (UA, angiogenesis),

Ron Lynch (UA, diabetes),

Sean Limesand (UA, diabetes),

Anne Cress (AZCC, prostate cancer),

Art Gmitro (Dept. of Radiology, esophagus cancer),

Scott Boitano (Bio5, astma),

Marilyn Halonen (UA, astma) ,

Michael Daines (UA, astma),

Jeff Walker (UA, cardiovascular),

Marty Pagel (UA, MRI).

Professional Information
Professional Affiliations: 

The American Chemical Society

The American Peptide Society

The Society of Combinatorial Sciences

Honors: 

2006          GI SPORE Career Development Award

1993-1995  Selectide (Sanofi-Aventis) Postdoctoral Fellowship

Academic Information
Doctorate: 
Institute of Pharmacy and Biochemistry, Prague, Czech Republic 1990
Master's Degree: 
Department of Organic Chemistry, Faculty of Chemical Technologies, The Institute of Technology, Prague, Czech Republic 1983