Multiplex Ligation-dependent Probe Amplifiation (MLPA)

Multiplex Ligation-dependent Probe Amplifiation (MLPA) is a high resolution method used to detect copy number variation in genomic sequences. Different kits are available for purchase from MRC Holland.

MLPA Procedure:

  1. Design probes to specficially target a single gene: each probe set varies in size by at least 1 base pair
  2. Bind probes to genomic DNA
  3. Ligate probes together to serve as template in PCR

  mlpa1.jpg

  1. Denature probes from target
  2. Amplify with universal primer
  3. Analyze like micro-satellite
  4. Normalize to set of known single copy probes

 mlpa2.jpg